Your Biohazard Waste Autoclave Procedure: What Time and Temperature?

When we last discussed red-bag waste autoclave procedure, we talked about running “your standard waste cycle” (noting that these vary by lab). So how do you determine your lab’s “standard” biohazard waste cycle?  The general rule for autoclave sterilization taught at most colleges is “15 minutes at 121ºC kills everything.“  But that’s definitely too short … Continue reading “Your Biohazard Waste Autoclave Procedure: What Time and Temperature?”

Autoclave Procedure for Pipette Tips

The autoclave procedure for pipette tips is a little more involved than other red bag waste. Why? Because pipette tips are pokey: They like to poke through bags and into people.  Even if your pipette tips themselves aren’t contaminated, they are a super-duper good vector for dangerous pathogens.  They’re also great at making sure goop … Continue reading “Autoclave Procedure for Pipette Tips”

Red-Bag Waste Autoclave Procedure

It’s a dirty job, but someone’s gotta do it.  Following this three-step autoclave procedure will result in reliable sterilization of “red bag” waste.  (Assuming that you aren’t running a BSL3 facility or disposing of animal carcasses.) Red Bag Waste Autoclave Procedure First things first, the DON’Ts: DON’T toss loose tips and pipettes into your biohazard … Continue reading “Red-Bag Waste Autoclave Procedure”

Lab Autoclave Function Primer: Intro to Air Ballast Systems

In general, autoclaving sealed flasks is a very, very bad practice.  But that isn’t to say it’s impossible.  If you need to sterilize sealed containers, then you need to make sure you have a special autoclave function to do it safely: an “air ballast” system. What’s an “Air Ballast” Autoclave Function? All steam autoclaves use … Continue reading “Lab Autoclave Function Primer: Intro to Air Ballast Systems”

Autoclave Procedure: Four Rules for No-Mess Liquid Sterilization

As we’ve discussed before, the best autoclave procedure for running liquid loads is trickier than just loading up the autoclave and choosing the “liquid cycle.” Follow these four rules and you’ll largely avoid trashing your autoclave chamber, plumbing, and drain while losing your liquids to evaporation, boil over, or burst bottles: 1. Never fill the bottles … Continue reading “Autoclave Procedure: Four Rules for No-Mess Liquid Sterilization”