Tissue Culture Cannabis Pushes Past the “Cutting Edge” of Cloning

“Cloning” sounds futuristic (and possibly sinister—especially when applied to sheep and monkeys).  But most of us did this in grade school: Take a rooted cutting from a “mother“ plant (for example, a chunk of raw potato that is sprouting an “eye”), plant it in soil, and you’ll grow a “clone” of that mother—a new plant, … Continue reading “Tissue Culture Cannabis Pushes Past the “Cutting Edge” of Cloning”

Types of Autoclaves: Choosing the Right Chamber

There are so many types of autoclaves — not just in terms of autoclave processes (dry heat, steam, etc.). There are also seemingly small differences—like size, orientation, and chamber type—that can have a big impact on your lab’s operations.  How do you determine which is right for your lab? This is a really interesting article … Continue reading “Types of Autoclaves: Choosing the Right Chamber”

Red-Bag Waste Autoclave Procedure

It’s a dirty job, but someone’s gotta do it.  Following this three-step autoclave procedure will result in reliable sterilization of “red bag” waste.  (Assuming that you aren’t running a BSL3 facility or disposing of animal carcasses.) Red Bag Waste Autoclave Procedure First things first, the DON’Ts: DON’T toss loose tips and pipettes into your biohazard … Continue reading “Red-Bag Waste Autoclave Procedure”

Autoclave Sterilization Tips: Abandon All Foil, Ye Who Pass This Way!

We need to talk about your autoclave sterilization rituals and aluminum foil. Specifically: Do not use aluminum foil when sterilizing empty beakers and flasks! Loosely crimping foil over the mouths of empty flasks prior to autoclaving them is a widespread practice in many research labs.  Justifications for this practice vary.  Some claim it keeps water … Continue reading “Autoclave Sterilization Tips: Abandon All Foil, Ye Who Pass This Way!”

Research Lab Autoclave Best Practices: The Liquid Cycle Won’t Save You

Every modern research lab autoclave has a “liquid cycle”—a cycle just for processing liquids, growth media, and so on.  Nonetheless, more often than not, if you fill a bunch of 1L bottles up to 900mL and process them on the “liquid cycle” they’re going to come out like this: The glass isn’t cracked; where did … Continue reading “Research Lab Autoclave Best Practices: The Liquid Cycle Won’t Save You”