Research Lab Autoclave Best Practices: The Liquid Cycle Won’t Save You

Every modern research lab autoclave has a “liquid cycle”—a cycle just for processing liquids, growth media, and so on.  Nonetheless, more often than not, if you fill a bunch of 1L bottles up to 900mL and process them on the “liquid cycle” they’re going to come out like this: The glass isn’t cracked; where did … Continue reading “Research Lab Autoclave Best Practices: The Liquid Cycle Won’t Save You”

Research Lab Autoclave Safety: Sealed Bottles are Bombs

In August of 2001 a research lab autoclave was processing tightly capped 178 bottles using a 30-minute liquid load.  The autoclave began making unusual sounds, so a trio of lab workers aborted the cycle and investigated.  They opened the autoclave to discover a few bottles had burst.  There was some lost liquid and a lot … Continue reading “Research Lab Autoclave Safety: Sealed Bottles are Bombs”

Research Lab Autoclave Safety: Bad Doors and Autoclave Explosions

  WARNING: These are disturbing pictures of research lab autoclave accidents. There is blood. What happened?  A lab tech loaded an older-style autoclave, began the cycle, and walked away to attend to other tasks.  Either due to user error or wear-and-tear over time, the door locking mechanism was not fully engaged.  As the cycle progressed, … Continue reading “Research Lab Autoclave Safety: Bad Doors and Autoclave Explosions”