Autoclave Procedure for Pipette Tips

The autoclave procedure for pipette tips is a little more involved than other red bag waste. Why? Because pipette tips are pokey: They like to poke through bags and into people.  Even if your pipette tips themselves aren’t contaminated, they are a super-duper good vector for dangerous pathogens.  They’re also great at making sure goop … Continue reading “Autoclave Procedure for Pipette Tips”

Red-Bag Waste Autoclave Procedure

It’s a dirty job, but someone’s gotta do it.  Following this three-step autoclave procedure will result in reliable sterilization of “red bag” waste.  (Assuming that you aren’t running a BSL3 facility or disposing of animal carcasses.) Red Bag Waste Autoclave Procedure First things first, the DON’Ts: DON’T toss loose tips and pipettes into your biohazard … Continue reading “Red-Bag Waste Autoclave Procedure”

Autoclaving Big Beakers and Flasks? Use the Vacuum Function!

If you’re regularly autoclaving large Erlenmeyer flasks, graduated cylinders, or beakers—and it’s not practical to run the entire load lying horizontally—a vacuum function is vital to your consistent success. Why Autoclaving Containers is a Hard Autoclaving any container is inherently challenging—even when that container is open.  Why?  Dense cold air tends to pool at the … Continue reading “Autoclaving Big Beakers and Flasks? Use the Vacuum Function!”

This No-Cost Lab Autoclave Procedure Tweak Significantly Improves Sterilization

We’ve spoken at length about the follies of foil, and regularly help customers kit out autoclaves precisely suited to their specific tasks.  But there is one thing that any lab can do at no cost— TODAY!—that will massively boost the effectiveness of their steam sterilizer: Autoclave large beakers, flasks, and cylinders on their sides—especially Erlenmeyer … Continue reading “This No-Cost Lab Autoclave Procedure Tweak Significantly Improves Sterilization”

Laboratory Autoclave Procedure Warning: Do You Account for “Thermal Inertia”?

Many labs don’t heat large liquid loads long enough to assure sterilization. The standard autoclave procedure is “hold a load at 121ºC for 20 minutes to assure sterilization.”  This is endorsed by everyone, from the United States Pharmacopeial Convention and Food & Drug Administration, to the Department of Health & Human Services and every university on … Continue reading “Laboratory Autoclave Procedure Warning: Do You Account for “Thermal Inertia”?”